Identification and characterisation of sugarcane intergeneric hybrids, Saccharum officinarum x Erianthus arundinaceus, with molecular markers and DNA in situ hybridisation
- 1 July 1995
- journal article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 91 (2), 320-326
- https://doi.org/10.1007/bf00220894
Abstract
Molecular markers were used to characterise sugarcane intergeneric hybrids between S. officinarum and E. arundinaceus. Very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged PCR. Two enzyme systems (GOT and MDH B) and PCR amplification revealing spacer-size variation in the 5s-rDNA cluster were found most convenient. Specific characterisation of the two genomic components was possible using RFLP and in situ hybridisation. The strong molecular differentiation between S. officinarum and E. arundinaceus allows the identification of numerous Erianthus-specific RFLP bands in the hybrids. Genomic DNA in situ hybridisation allows for the differentiation of the chromosomes contributed by S. officinarum and E. arundinaceus in chromosome preparations of the hybrids. In situ hybridisation with the 18s-5.8s-25s rDNA probe highlights the basic chromosome numbers in the two parental species. The potential of these techniques to monitor the Erianthus genome during the introgression process is discussed.Keywords
This publication has 15 references indexed in Scilit:
- A molecular approach to unraveling the genetics of sugarcane, a complex polyploid of the Andropogoneae tribeGenome, 1994
- Phylogenetic analysis of chloroplast restriction enzyme site mutations in the Saccharinae Griseb. subtribe of the Andropogoneae Dumort. tribeTheoretical and Applied Genetics, 1994
- Alkali treatment for rapid preparation of plant material for reliable PCR analysisThe Plant Journal, 1993
- Reprobing of DNA: DNA in situ hybridization preparationsTrends in Genetics, 1992
- Use of the polymerase chain reaction to detect spacer size heterogeneity in plant 5S-rRNA gene clusters and to locate such clusters in wheat (Triticum aestivum L.)Theoretical and Applied Genetics, 1992
- Relationships betweenOryza species (Poaceae) based on 5S DNA sequencesÖsterreichische botanische Zeitschrift, 1992
- The 5S RNA genes inPinus radiata and the spacer region as a probe for relationships betweenPinus speciesÖsterreichische botanische Zeitschrift, 1992
- Organization and evolution of the 5S ribosomal RNA gene family in wheat and related speciesGenome, 1989
- Biochemical genetic markers in sugarcaneTheoretical and Applied Genetics, 1989
- Starch gel electrophoresis of enzymes?A compilation of recipesBiochemical Genetics, 1970