Polyoma middle tumor antigen interacts with SHC protein via the NPTY (Asn-Pro-Thr-Tyr) motif in middle tumor antigen.
- 5 July 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 91 (14), 6344-6348
- https://doi.org/10.1073/pnas.91.14.6344
Abstract
Polyomavirus middle tumor antigen (MT) transforms a large number of cell types by binding to and modulating the activities of cellular proteins. Previous genetic analysis defined in MT an independent motif, NPTY (Asn-Pro-Thr-Tyr), required for transformation. This report demonstrates that NPTY is required for interaction between MT and SHC protein, a Src homology 2 (SH2)-containing protooncogene product implicated in activating Ras via association with GRB2 protein. SHC is phosphorylated on tyrosine and associates with GRB2 in MT-transformed cells. These effects require an intact NPTY motif in MT. SHC immunoprecipitates from MT-transformed cells possess kinase activity that phosphorylates not only SHC and MT but also the 85-kDa subunit of phosphatidylinositol 3-kinase. This result suggests that a complex exists that contains, at a minimum, MT, Src family tyrosine kinases, phosphatidylinositol 3-kinase, and SHC.Keywords
This publication has 36 references indexed in Scilit:
- SH2 domains recognize specific phosphopeptide sequencesCell, 1993
- Oncogenes, Growth Factors, and Signal TransductionNew England Journal of Medicine, 1989
- Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activityCell, 1987
- An 81 kd protein complexed with middle T antigen and pp60c-src: A possible phosphatidylinositol kinaseCell, 1987
- Association of the polyomavirus middle-T antigen with c-yes proteinNature, 1987
- Enhancement of cellular src gene product associated tyrosyl kinase activity following polyoma virus infection and transformationCell, 1984
- Polyoma virus transforming protein associates with the product of the c-src cellular geneNature, 1983
- Phosphorylation of polyoma T antigensCell, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970