Primary structure and expression of a functional human glucocorticoid receptor cDNA

Abstract

Identification of complementary DNAs encoding the human glucocorticoid receptor predicts two protein forms, of 777 (α) and 742 (β) amino acids, which differ at their carboxy termini. The proteins contain a cysteine/lysine/arginine-rich region which may define the DNA-binding domain. Pure radiolabelled glucocorticoid receptor, synthesized in vitro, is immuno-reactive and possesses intrinsic steroid-binding activity characteristic of the native glucocorticoid receptor.