Cybrd1 (duodenal cytochrome b) is not necessary for dietary iron absorption in mice
Open Access
- 15 October 2005
- journal article
- Published by American Society of Hematology in Blood
- Vol. 106 (8), 2879-2883
- https://doi.org/10.1182/blood-2005-02-0716
Abstract
Mammalian nonheme iron absorption requires reduction of dietary iron for uptake by the divalent metal ion transport system in the intestine. This was thought to be mediated by duodenal cytochrome b (Cybrd1), a ferric reductase enzyme resident on the luminal surface of intestinal absorptive cells. To test its importance in vivo, we inactivated the murine Cybrd1 gene and assessed tissue iron stores in Cybrd1-null mice. We found that loss of Cybrd1 had little or no impact on body iron stores, even in the setting of iron deficiency. We conclude that other mechanisms must be available for the reduction of dietary iron. (Blood. 2005;106: 2879-2883)Keywords
This publication has 17 references indexed in Scilit:
- Tissue-specific changes in iron metabolism genes in mice following phenylhydrazine-induced haemolysisBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2004
- Changes in the expression of intestinal iron transport and hepatic regulatory molecules explain the enhanced iron absorption associated with pregnancy in the ratGut, 2004
- Duodenal Ascorbate Levels Are Changed in Mice with Altered Iron MetabolismJournal of Nutrition, 2004
- Iron overload in adult Hfe-deficient mice independent of changes in the steady-state expression of the duodenal iron transporters DMT1 and Ireg1/ferroportinJournal of Molecular Medicine, 2003
- Regulatory defects in liver and intestine implicate abnormal hepcidin and Cybrd1 expression in mouse hemochromatosisNature Genetics, 2003
- Inappropriate expression of hepcidin is associated with iron refractory anemia: implications for the anemia of chronic diseaseBlood, 2002
- An Iron-Regulated Ferric Reductase Associated with the Absorption of Dietary IronScience, 2001
- Ascorbate Stimulates Ferricyanide Reduction in HL-60 Cells through a Mechanism Distinct from the NADH-dependent Plasma Membrane ReductaseJournal of Biological Chemistry, 1998
- Characterization and partial purification of a ferrireductase from human duodenal microvillus membranesBiochemical Journal, 1995
- Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1990