Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review
Top Cited Papers
- 27 March 2012
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 12 (14), 2469-2486
- https://doi.org/10.1039/c2lc40100b
Abstract
Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of diagnostic devices will interrogate the genetic determinants of such conditions at the point-of-care, affording clinicians prompt reliable diagnosis from which to guide more effective treatment. The complex biochemical nature of clinical samples, the low abundance of nucleic acid targets in the majority of clinical samples and existing biosensor technology indicate that some form of nucleic acid amplification will be required to obtain clinically relevant sensitivities from the small samples used in point-of-care testing (POCT). This publication provides an overview and thorough review of existing technologies for nucleic acid amplification. The different methods are compared and their suitability for POCT adaptation are discussed. Current commercial products employing isothermal amplification strategies are also investigated. In conclusion we identify the factors impeding the integration of the methods discussed in fully automated, sample-to-answer POCT devices.Keywords
This publication has 162 references indexed in Scilit:
- Advances in microfluidic PCR for point-of-care infectious disease diagnosticsBiotechnology Advances, 2011
- Molecular Methods and Platforms for Infectious Diseases Testing: A Review of FDA-Approved and Cleared AssaysThe Journal of Molecular Diagnostics, 2011
- Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChipAnalytical Chemistry, 2011
- Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 VirusThe Journal of Molecular Diagnostics, 2011
- Nucleic Acid Assay System for Tier II Laboratories and Moderately Complex Clinics to Detect HIV in Low‐Resource SettingsThe Journal of Infectious Diseases, 2010
- Simple and accurate determination of CYP2D6 gene copy number by a loop-mediated isothermal amplification method and an electrochemical DNA chipClinica Chimica Acta; International Journal of Clinical Chemistry, 2010
- An integrated disposable device for DNA extraction and helicase dependent amplificationBiomedical Microdevices, 2010
- Dry cotton or flocked respiratory swabs as a simple collection technique for the molecular detection of respiratory viruses using real-time NASBAJournal of Virological Methods, 2008
- Loop-mediated isothermal amplification of a single DNA molecule in polyacrylamide gel-based microchamberBiomedical Microdevices, 2008
- Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplificationNucleic Acids Research, 2006