Molecular characterization of a gene encoding a photolyase fromStreptomycesgriseus
- 1 January 1989
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (12), 4731-4744
- https://doi.org/10.1093/nar/17.12.4731
Abstract
By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of Streptomyces griseus. One ORF encodes a polypeptide of 455 amino acids (Mr 50594), which exhibits substantial similarities with the other three photolyases. Photoreactivation-repair deficient E. coli cells could be converted into photoreactivatable ones by introduction of plasmids harboring this ORF, indicating that this is the photolyase gene of S. griseus. The deduced aa sequence of Streptomyces photolyase was most similar to that of E. coli. The putative DNA binding site as well as cofactor binding regions were proposed.Keywords
This publication has 22 references indexed in Scilit:
- Identification of a pterin derivative in Escherichia coli DNA photolyaseBiochemistry, 1988
- DNA REPAIR ENZYMESAnnual Review of Biochemistry, 1988
- PHOTOREACTIVATING ENZYME FROM Streptomyces griseus—VI. ACTION SPECTRUM AND KINETICS OF PHOTOREACTIVATIONPhotochemistry and Photobiology, 1986
- Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coliGene, 1985
- The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequencesGene, 1984
- PROTEIN-DNA RECOGNITIONAnnual Review of Biochemistry, 1984
- Escherichia coli DNA photolyase is a flavoproteinJournal of Molecular Biology, 1984
- Evidence that deoxyribonucleic acid photolyase from bakers' yeast is a flavoproteinBiochemistry, 1980
- Prediction of the Secondary Structure of Proteins from their Amino Acid SequencePublished by Wiley ,1979
- Cloning of the phr gene and amplification of photolyase in Escherichia coliGene, 1978