A highly efficient rare earth metal oxide nanorods based platform for aflatoxin detection
- 3 July 2013
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Materials Chemistry B
- Vol. 1 (35), 4493-4503
- https://doi.org/10.1039/c3tb20690d
Abstract
The nanostructured rare earth metal oxide (samarium oxide, n-Sm2O3) nanorods, prepared using a forced hydrolysis technique, have been electrophoretically deposited (EPD) onto an indium-tin-oxide (ITO) glass substrate. This novel platform has been utilized for co-immobilization of monoclonal antibodies of aflatoxin B1 (Ab-AFB1) and bovine serum albumin (BSA) via electrostatic interactions for food toxin (AFB1) detection. Thus prepared n-Sm2O3 nanorods have been characterized by X-ray diffraction (XRD), atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopic techniques. The results of electrochemical response studies of the BSA/Ab-AFB1/n-Sm2O3/ITO immunoelectrode obtained as a function of aflatoxin concentration reveal a linearity of 10–700 pg mL−1, a detection limit of 57.82 pg mL−1 cm−2, a response time of 5 s and a sensitivity of 48.39 μA pg−1 mL−1 cm−2 with a regression coefficient of 0.961. The association constant (Ka) for antigen–antibody interactions obtained is 47.9 pg mL−1, which indicates high affinity of antibodies towards the antigen (AFB1). The application of n-Sm2O3 modified electrode for immunosensor analysis offers a novel platform and efficient strategy for the application of rare earth metal oxide materials in bioelectronics.Keywords
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