Normal mouse kidneys contain activated and CD3+CD4−CD8− double-negative T lymphocytes with a distinct TCR repertoire
Open Access
- 2 September 2008
- journal article
- pathogenesis and-host-defense
- Published by Oxford University Press (OUP) in Journal of Leukocyte Biology
- Vol. 84 (6), 1400-1409
- https://doi.org/10.1189/jlb.0907651
Abstract
Healthy liver, intestine, lung, and skin harbor resident lymphocytes with conventional and unconventional phenotypes. Lymphocytes also have been detected in healthy mice kidneys; however, these cells have not been well studied and have been largely overlooked. To better characterize the intra‐renal lymphocytes, we extensively perfused C57BL/6J mice with PBS and then isolated mononuclear cells for flow cytometry analysis. We observed T cells, B cells, and NK cells in normal mice kidneys after extensive perfusion. Approximately 50% of kidney T lymphocytes expressed intermediate levels of CD3 (CD3int T cells). Similar to liver and lung, a high percentage of unconventional CD3+CD4−CD8− double‐negative T cells was observed in normal mice kidneys, from which 11% expressed B220 antigen. Unlike the spleen and blood, the classic CD4+ and CD8+ T lymphocytes in the kidney had a high proportion of activated CD69+ and effector/memory CD44CD62L ligand phenotypes. Also, a small percentage of CD4+CD25+forkhead box p3+ and NKT cells was observed in perfused and exanguinated kidneys. In addition, a distinct TCR repertoire was found on intra‐renal conventional and unconventional T cells compared with those from the spleen. Finally, after 24 h of renal ischemia reperfusion injury (IRI), increased production of cytokines IFN‐γ and TNF‐α by CD4+ and CD8+ T cells, isolated from perfused kidneys, was observed. These data suggest that some of these cells harbored in the kidney could be implicated in the immune response of the IRI pathogenic process.Keywords
Funding Information
- National Institutes of Health (R01 DK54770, 3R01DK054770-05S1, 3R01DK054770-06A1, SCCOR HL073944, RO1 AI42287, R21 AI063133)
This publication has 41 references indexed in Scilit:
- Variation in numbers of CD4+CD25highFOXP3+T cells with normal immuno-regulatory properties in long-term graft outcomeTransplant International, 2007
- Lymphocyte function during hepatic ischemia/reperfusion injuryJournal of Leukocyte Biology, 2007
- Role of T Lymphocytes and Interferon-γ in Ischemic StrokeCirculation, 2006
- INTESTINAL AND PULMONARY MUCOSAL T CELLS: Local Heroes Fight to Maintain the Status QuoAnnual Review of Immunology, 2006
- Association of NKT cells and granulocytes with liver injury after reperfusion of the portal veinCellular Immunology, 2005
- The composition of intrahepatic lymphocytes: shaped by selective recruitment?Trends in Immunology, 2004
- Hepatoimmunology: A perspectiveImmunology & Cell Biology, 2002
- The liver as a site of T-cell apoptosis: graveyard, or killing field?Immunological Reviews, 2000
- Isolation, phenotyping, and functional analysis of lymphocytes from human liverClinical Immunology and Immunopathology, 1990
- ANALYSIS OF THE EFFECTOR FUNCTIONS OF DIFFERENT POPULATIONS OF MUCOSAL LYMPHOCYTES*Annals of the New York Academy of Sciences, 1983