Differential diagnosis of bladder versus colorectal adenocarcinoma: keratin 7 and GATA3 positivity in nuclear ß-catenin-negative glandular tumours defines adenocarcinoma of the bladder
- 13 October 2015
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 69 (4), 307-312
- https://doi.org/10.1136/jclinpath-2015-203144
Abstract
Aims This study evaluates immunohistochemical markers for the differential diagnosis of primary bladder adenocarcinoma (BAC) from secondarily involving colorectal adenocarcinoma (CAC). Additional staining of putative precursor lesions (cystitis cystica et glandularis (CC) and intestinal metaplasia (IM)) supports insights into metaplastic cell development and aberrant differentiation in tumours. Methods Tissue microarray sections of formalin-fixed, paraffin-embedded tissues from clinically verified 11 BAC, 11 CAC, 18 invasive urothelial carcinomas (UCs), 22 normal urothelium samples, 25 CC and 15 IM were stained for keratin 7, 5/6, 5/14 and 20, ß-catenin, e-cadherin, cadherin 17, cdx2, uroplakin II and III, CD10, androgen receptor (AR), S100P, MUC2, MUC5AC and GATA3 expression. Data were analysed using Kruskal–Wallis/Dunn's multiple comparison test and Fisher's exact test. Results Significant difference (pConclusions Differential diagnosis of BAC and CAC remains difficult, but positive staining for keratin 7 in nuclear ß-catenin-negative tumours argues for BAC. Additional markers like GATA3 and p63 may be added, as positivity in some cases may be helpful. However, for reliable histological diagnosis, knowledge of comprehensive clinical data is still essential.Keywords
This publication has 22 references indexed in Scilit:
- Metaplastic conditions of the bladderBJU International, 2013
- Distinguishing primary adenocarcinoma of the urinary bladder from secondary involvement by colorectal adenocarcinoma: extended immunohistochemical profiles emphasizing novel markersLaboratory Investigation, 2013
- p63-expressing cells are the stem cells of developing prostate, bladder, and colorectal epitheliaProceedings of the National Academy of Sciences of the United States of America, 2013
- Primary bladder adenocarcinoma versus metastatic colorectal adenocarcinoma: a persisting diagnostic challengeDiagnostic Pathology, 2012
- S100P, a potential novel prognostic marker in colorectal cancerOncology Reports, 2012
- Role of Cystitis Cystica et Glandularis and Intestinal Metaplasia in Development of Bladder CarcinomaUrology, 2008
- The human keratins: biology and pathologyHistochemistry and Cell Biology, 2008
- Placental S100 (S100P) and GATA3: Markers for Transitional Epithelium and Urothelial Carcinoma Discovered by Complementary DNA MicroarrayThe American Journal of Surgical Pathology, 2007
- Value of CDX2, villin, and α-methylacyl coenzyme A racemase immunostains in the distinction between primary adenocarcinoma of the bladder and secondary colorectal adenocarcinomaLaboratory Investigation, 2005
- Uroplakin III Is a Highly Specific and Moderately Sensitive Immunohistochemical Marker for Primary and Metastatic Urothelial CarcinomasAmerican Journal of Clinical Pathology, 2000