Molecular Imprinting-Chemiluminescence Sensor for the Determination of Amoxicillin
- 11 March 2010
- journal article
- spectroscopy
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 43 (6), 1033-1045
- https://doi.org/10.1080/00032710903491104
Abstract
The amoxicillin-imprinted polymer was synthesized with methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. The binding characteristic of the imprinted polymer to amoxicillin was evaluated by equilibrium binding experiments. Using the imprinted polymer as recognition material, 3-(3′-nitrophenyl)-5(2′-sulfonylphenylazo)-rhodanine (4NRASP) was synthesized by the authors and was used as chemiluminescence (CL) reagent. A novel chemiluminescence (CL) sensor for the determination of amoxicillin was developed based on the CL reaction of amoxicillin with potassium permanganate in an acidic medium. The sensor displayed excellent selectivity and high sensitivity. The linear response range of the sensor was from 5.0 × 10−9 to 1.0 × 10−6 g · mL−1 (r = 0.9985) and the detection limit was 1.3 × 10−9 g · mL−1. The relative standard deviation for the determination of 1.0 × 10−7 g · mL−1 amoxicillin solution was 1.7% (n = 11). The sensor was applied to the determination of amoxicillin in urine samples with satisfactory results.Keywords
This publication has 12 references indexed in Scilit:
- Flow Injection Chemiluminescence Sensor with Novel Rhodanine Ramification for Determination of Fenfluramine Based on Molecularly Imprinted PolymerAnalytical Letters, 2009
- Catalytic dynamic spectrofluorimetry determination of trace antimony using new type arsenoxylphenylazo rhodanineSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2009
- Speciation Analysis of Organotin Compounds in Shellfish by Hyphenated Technique of High Performance Liquid Chromatography-Inductively Coupled Plasma Mass SpectrometryChinese Journal of Analytical Chemistry, 2008
- Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymerBiosensors and Bioelectronics, 2008
- Determination of fenfluramine based on potassium permanganate–calcein chemiluminescence systemTalanta, 2008
- Simultaneous determination of amoxicillin and clavulanic acid in human plasma by isocratic reversed-phase HPLC using UV detectionJournal of Pharmaceutical and Biomedical Analysis, 2007
- Molecularly imprinted microspheres and nanospheres for di(2-ethylhexyl)phthalate prepared by precipitation polymerizationAnalytical and Bioanalytical Chemistry, 2007
- Functional Mimicry of the Active Site of Carboxypeptidase A by a Molecular Imprinting Strategy: Cooperativity of an Amidinium and a Copper Ion in a Transition-State Imprinted Cavity Giving Rise to High Catalytic ActivityJournal of the American Chemical Society, 2004
- Polyacrylamide gels with electrostatic functional groups for the molecular imprinting of lysozymeAnalytica Chimica Acta, 2004
- Enzyme-like Catalysis by Molecularly Imprinted PolymersChemical Reviews, 2001