MALDI-TOF mass spectrometry as a tool for differentiation of invasive and noninvasiveStreptococcus pyogenesisolates
Open Access
- 1 August 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Immunology & Medical Microbiology
- Vol. 53 (3), 333-342
- https://doi.org/10.1111/j.1574-695x.2008.00428.x
Abstract
A novel mass spectral fingerprinting and proteomics approach using MALDI-TOF MS was applied to detect and identify protein biomarkers of group A Streptococcus (GAS) strains. Streptococcus pyogenes ATCC 700294 genome strain was compared with eight GAS clinical isolates to explore the ability of MALDI-TOF MS to differentiate isolates. Reference strains of other bacterial species were also analyzed and compared with the GAS isolates. MALDI preparations were optimized by varying solvents, matrices, plating techniques, and mass ranges for S. pyogenes ATCC 700294. Spectral variability was tested. A subset of common, characteristic, and reproducible biomarkers in the range of 2000–14 000 Da were detected, and they appeared to be independent of the culture media. Statistical analysis confirmed method reproducibility. Random Forest analysis of all selected GAS isolates revealed differences among most of them, and summed spectra were used for hierarchical cluster analysis. Specific biomarkers were found for each strain, and invasive GAS isolates could be differentiated. GAS isolates from cases of necrotizing fasciitis were clustered together and were distinct from isolates associated with noninvasive infections, despite their sharing the same emm type. Almost 30% of the biomarkers detected were tentatively identified as ribosomal proteins.Keywords
This publication has 42 references indexed in Scilit:
- Universal Sample Preparation Method for Characterization of Bacteria by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass SpectrometryApplied and Environmental Microbiology, 2007
- Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass SpectrometryJournal of Clinical Microbiology, 2006
- Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A StreptococcusProceedings of the National Academy of Sciences of the United States of America, 2006
- Effects of varied pH, growth rate and temperature using controlled fermentation and batch culture on Matrix Assisted Laser Desorption/Ionization whole cell protein fingerprintsJournal of Microbiological Methods, 2005
- Comparison of emm Typing and Ribotyping with Three Restriction Enzymes To Characterize Clinical Isolates of Streptococcus pyogenesJournal of Clinical Microbiology, 2005
- MALDI-TOFMS compared with other polyphasic taxonomy approaches for the identification and classification of Bacillus pumilus sporesJournal of Microbiological Methods, 2004
- Mass spectrometry-based proteomicsNature, 2003
- Genetic and Phenotypic Features of Streptococcus pyogenes Strains Isolated in Brazil That Harbor New emm SequencesJournal of Clinical Microbiology, 2001
- Characterization of intact microorganisms by MALDI mass spectrometryMass Spectrometry Reviews, 2001
- emmTyping and Validation of Provisional M Types for Group A Streptococci1Emerging Infectious Diseases, 1999