Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation
- 1 June 2001
- journal article
- Published by Optica Publishing Group in Optics Letters
- Vol. 26 (11), 828-830
- https://doi.org/10.1364/ol.26.000828
Abstract
A method for increasing lateral as well as axial resolution in fluorescence microscopy is presented. A passband with a high cutoff frequency throughout reciprocal space can be achieved by illumination of the object with spatially harmonic excitation patterns generated by the interference of two collimated laser beams. Theoretical calculations show an almost isotropic point-spread function with a FWHM near 100 nm.Keywords
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