Complex formation between RAS and RAF and other protein kinases.
- 1 July 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (13), 6213-6217
- https://doi.org/10.1073/pnas.90.13.6213
Abstract
We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.Keywords
This publication has 50 references indexed in Scilit:
- Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein.Genes & Development, 1992
- Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.Genes & Development, 1992
- Serum-, TPA-, and Ras-induced expression from Ap-1/Ets-driven promoters requires Raf-1 kinase.Genes & Development, 1992
- ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSKCell, 1992
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- ras GENESAnnual Review of Biochemistry, 1987
- Requirement for c-ras proteins during viral oncogene transformationNature, 1986
- Differential activation of yeast adenylate cyclase by wild type and mutant RAS proteinsCell, 1985
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984