Immunochemical Determination of 2,4,6-Trichloroanisole as the Responsible Agent for the Musty Odor in Foods. 2. Immunoassay Evaluation
- 3 June 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 51 (14), 3932-3939
- https://doi.org/10.1021/jf034001x
Abstract
Immunoassays for 2,4,6-trichloroanisol (TCA) have been evaluated. The assays were developed after raising antibodies against three different immunizing haptens ( 1). Lack of reproducibility has been one of the main problems of these assays. Precision was worse on these assays, reaching lower limits of detection. The high lipophilicity of TCA and its, consequently, low water solubility have been found to be the major cause of this problem. A reliable microplate-based enzyme-linked immunosorbent assay (ELISA) has been set after consideration of the TCA physicochemical features and evaluation of important parameters affecting immunoassay performance. The immunoassay uses As78 (developed against hapten B-KLH) and C9-OVA as the coating antigen. The selectivity is high although the brominated analogue 2,4,6-TBA is also recognized. In buffered media containing 7% ethanol, the resulting assay shows a good accuracy with an IC50 value of 0.53 μg L-1 and a limit of detection of 0.044 μg L-1. Red and white wine samples caused important interferences in the immunoassay demonstrating the necessity of a cleanup procedure prior to the ELISA. Keywords: Trichloroanisole; musty odor; wine; cork; immunoassay; lipophilicityKeywords
This publication has 21 references indexed in Scilit:
- Applications and Recent Developments in the use of Antibodies for AnalysisAnalytical Letters, 2000
- Current Trends in Immunoassay-Based Kits for Pesticide AnalysisCritical Reviews in Food Science and Nutrition, 1999
- Preparation of antisera and development of a direct enzyme-linked immunosorbent assay for the determination of the antifouling agent Irgarol 1051Analytica Chimica Acta, 1997
- Development of a highly sensitive enzyme-linked immunosorbent assay for atrazine Performance evaluation by flow injection immunoassayAnalytica Chimica Acta, 1997
- Charge transfer devices in spectroscopy,: edited by Jonathan V. Sweedler, Kenneth L. Ratzlaff and M. Bonner Denton, VCH, New York, 406 pp., 1994, US$ 95, ISBN 1-56081-060-2TrAC Trends in Analytical Chemistry, 1995
- Chlorinated anisoles and veratroles in a Canadian river receiving bleached kraft pulp mill effluent. Identification, distribution, and olfactory evaluationEnvironmental Science & Technology, 1993
- Increased sensitivity of an enzyme immunoassay (ELISA) for the determination of triazine herbicides by variation of tracer incubation timeMicrochimica Acta, 1992
- An immunoassay for pyrethroids: detection of permethrin in meatJournal of Agricultural and Food Chemistry, 1989
- Simultaneous determination of 2,4,6‐trichloroanisole, 2,3,4,6‐tetrachloroanisole and pentachloroanisole in foods and packaging materials by high‐resolution gas chromatography‐multiple ion monitoring‐mass spectrometryJournal of the Science of Food and Agriculture, 1986
- Identification of 2,4,6-trichloroanisole as a potent compound causing cork taint in wineJournal of Agricultural and Food Chemistry, 1982