A thermostable sequence-specific endonuclease from Thermus aquaticus.
- 1 February 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 74 (2), 542-546
- https://doi.org/10.1073/pnas.74.2.542
Abstract
A sequence-specific endonuclease, Taq I, of novel specificity was partially purified from an extreme thermophile, T. aquaticus. The enzyme cleaves bacteriophage .lambda. DNA at many (> 30) sites and bacteriophage .vphi.X174 RF [replicative form] DNA at 10 sites. The enzyme is active at temperatures up to 70.degree. C. The cleavage sites on .vphi.X174 RF DNA were mapped. The sequence recognized and cleaved by Taq I was the symmetrical tetranucleotide: .**GRAPHIC**.This publication has 12 references indexed in Scilit:
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