DNA as a Dietary Biomarker in Antarctic Krill, Euphausia superba
- 23 August 2006
- journal article
- Published by Springer Science and Business Media LLC in Marine Biotechnology
- Vol. 8 (6), 686-696
- https://doi.org/10.1007/s10126-005-6088-8
Abstract
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNA-based estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods.Keywords
This publication has 39 references indexed in Scilit:
- DNA Barcoding: Error Rates Based on Comprehensive SamplingPLoS Biology, 2005
- TaxI: a software tool for DNA barcoding using distance methodsPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2005
- Molecular scatology as a tool to study diet: analysis of prey DNA in scats from captive Steller sea lionsMolecular Ecology, 2005
- On the use of lipid biomarkers in marine food web analyses: An experimental case study on the Antarctic krill, Euphausia superbaLimnology and Oceanography, 2003
- The correlation between rDNA copy number and genome size in eukaryotesGenome, 2003
- A Greedy Algorithm for Aligning DNA SequencesJournal of Computational Biology, 2000
- Krill-copepod interactions at South Georgia, Antarctica, I. Omnivory by Euphausia superbaMarine Ecology Progress Series, 1997
- Energetics and feeding dynamics of Euphausia superba in the South Georgia region during the summer of 1994Journal of Plankton Research, 1997
- Behavioral and Physiological Characteristics of the Antarctic Krill,Euphausia superbaAmerican Zoologist, 1991
- Basic local alignment search toolJournal of Molecular Biology, 1990