Survey Methods for Assessment of Citrus Tristeza Virus Incidence
Open Access
- 1 July 1998
- journal article
- Published by Scientific Societies in Phytopathology®
- Vol. 88 (7), 715-723
- https://doi.org/10.1094/phyto.1998.88.7.715
Abstract
The assessment of citrus tristeza virus incidence by sampling involves laboratory testing by enzyme-linked immunosorbent assay of leaf material collected in the field. Using field data and computer simulation, methods of field data collection were compared. One method was similar to that used by the Central California Tristeza Eradication Agency, in which 4 to 6% of the trees in a planting block are sampled and material from each tree sampled is assayed separately. This method was compared with an alternative method in which about 25% of the trees in a block are sampled, and material from groups of four trees is bulked and assayed together. Our comparative study indicated that the latter method results in increased accuracy and precision of estimates of citrus tristeza virus incidence without increasing unduly the number of laboratory assays required.Keywords
This publication has 24 references indexed in Scilit:
- Investigating Mechanisms of Spatiotemporal Epidemic Spread Using Stochastic ModelsPhytopathology®, 1997
- Plant disease incidence: inverse sampling, sequential sampling, and confidence intervals when observed mean incidence is zeroCrop Protection, 1996
- Spatial and Temporal Analyses of Citrus Tristeza Virus in Eastern SpainPhytopathology®, 1996
- Sampling for detection: Leek rust as an exampleInternational Journal of Pest Management, 1995
- Citrus Tristeza Virus and Its Aphid VectorToxoptera citmicida: Threats to Citrus Production in the Caribbean and Central and North AmericaPlant Disease, 1995
- The use of a sequential batch testing procedure and ELISA to determine the incidence of five viruses in Victorian cut-flower Sim carnationsAustralian Journal of Agricultural Research, 1994
- Transmission of Exotic Citrus Tristeza Virus Isolates by a Florida Colony of Aphis gossypiiPlant Disease, 1989
- Group Testing for Estimating Infection Rates and Probabilities of Disease TransmissionPhytopathology®, 1985
- AN EXAMINATION OF FIVE SAMPLING METHODS UNDER RANDOM AND CLUSTERED DISEASE DISTRIBUTIONS USING SIMULATIONCanadian Journal of Plant Science, 1979
- Estimation of the Proportion of Vectors in a Natural Population of InsectsPublished by JSTOR ,1962