NEDD1-dependent recruitment of the γ-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly
Open Access
- 6 February 2006
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 172 (4), 505-515
- https://doi.org/10.1083/jcb.200510028
Abstract
The centrosome is the major microtubule organizing structure in somatic cells. Centrosomal microtubule nucleation depends on the protein γ-tubulin. In mammals, γ-tubulin associates with additional proteins into a large complex, the γ-tubulin ring complex (γTuRC). We characterize NEDD1, a centrosomal protein that associates with γTuRCs. We show that the majority of γTuRCs assemble even after NEDD1 depletion but require NEDD1 for centrosomal targeting. In contrast, NEDD1 can target to the centrosome in the absence of γ-tubulin. NEDD1-depleted cells show defects in centrosomal microtubule nucleation and form aberrant mitotic spindles with poorly separated poles. Similar spindle defects are obtained by overexpression of a fusion protein of GFP tagged to the carboxy-terminal half of NEDD1, which mediates binding to γTuRCs. Further, we show that depletion of NEDD1 inhibits centriole duplication, as does depletion of γ-tubulin. Our data suggest that centriole duplication requires NEDD1-dependent recruitment of γ-tubulin to the centrosome.Keywords
This publication has 58 references indexed in Scilit:
- Gene knockout analysis of two γ-tubulin isoforms in miceDevelopmental Biology, 2005
- De novo formation of basal bodies in Naegleria gruberi The Journal of cell biology, 2005
- SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cellsNature, 2005
- Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in γ-tubulin-depleted cellsJournal of Cell Science, 2004
- The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAKThe Journal of cell biology, 2004
- Proteomic characterization of the human centrosome by protein correlation profilingNature, 2003
- Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cellsThe Journal of cell biology, 2003
- SAS-4 Is a C. elegans Centriolar Protein that Controls Centrosome SizeCell, 2003
- Characterization of the Human Homologue of the Yeast Spc98p and Its Association with γ-TubulinThe Journal of cell biology, 1998
- Identification of a set of genes with developmentally down-regulated expression in the mouse brainBiochemical and Biophysical Research Communications, 1992