Enolase takes part in a macromolecular complex associated to mitochondria in yeast
- 31 October 2006
- journal article
- Published by Elsevier BV in Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Vol. 1757 (9-10), 1217-1228
- https://doi.org/10.1016/j.bbabio.2006.07.001
Abstract
In eucaryotes, glycolytic enzymes are classically regarded as being localised in the cytosol. Recently, we have shown that part of the cellular pool of the glycolytic enzyme, enolase, is tightly associated with the mitochondrial surface in the yeast Saccharomyces cerevisiae (N. Entelis, I. Brandina, P. Kamenski, I.A. Krasheninnikov, R.P. Martin and I. Tarassov, A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae, Genes Dev. 20 (2006) 1609-1620). Here, using enzymatic assays, we show that all glycolytic enzymes are associated with mitochondria in yeast, to extents similar to those previously reported for Arabidopsis cells. Using separation of mitochondrial complexes by blue-native/SDS-PAGE and coimmunoprecipitation of mitochondrial proteins with anti-enolase antibodies, we found that enolase takes part in a large macromolecular complex associated to mitochondria. The identified components included additional glycolytic enzymes, mitochondrial membrane carriers, and enzymes of the TCA cycle. We suggest a possible role of the enolase complex in the channeling of pyruvate, the terminal product of glycolysis, towards the TCA cycle within mitochondria. Moreover, we show that the mitochondrial enolase-containing complex also contains the cytosolic tRNACUULys, which is mitochondrially-imported, and its presumed import carrier, the precursor of the mitochondrial lysyl-tRNA synthetase. This suggests an unsuspected novel function for this complex in tRNA mitochondrial import.Keywords
This publication has 43 references indexed in Scilit:
- A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiaeGenes & Development, 2006
- Saccharomyces cerevisiae imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrionGenes & Development, 2005
- Integrative Analysis of the Mitochondrial Proteome in YeastPLoS Biology, 2004
- Identification of the mitochondrial pyruvate carrier in Saccharomyces cerevisiaeBiochemical Journal, 2003
- Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free‐flow electrophoresisProteomics, 2003
- Functional organization of the yeast proteome by systematic analysis of protein complexesNature, 2002
- Yeast Mitochondrial Dehydrogenases Are Associated in a Supramolecular ComplexBiochemistry, 2001
- An Intact Protein Translocating Machinery is Required for Mitochondrial Import of a Yeast Cytoplasmic tRNAJournal of Molecular Biology, 1995
- Cross-linking analysis of yeast mitochondrial outer membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- Escherichia coli citrate synthase. Purification and the effect of potassium on some propertiesBiochemistry, 1969