Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples
- 1 December 2012
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 50 (12), 4147-4150
- https://doi.org/10.1128/jcm.02659-12
Abstract
The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and −80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets ( P < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples.Keywords
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