Roles for wbtC, wbtI, and kdtA Genes in Lipopolysaccharide Biosynthesis, Protein Glycosylation, Virulence, and Immunogenicity in Francisella tularensis Strain SCHU S4
Open Access
- 9 September 2012
- Vol. 1 (1), 12-29
- https://doi.org/10.3390/pathogens1010012
Abstract
Using a strategy of gene deletion mutagenesis, we have examined the roles of genes putatively involved in lipopolysaccharide biosynthesis in the virulent facultative intracellular bacterial pathogen, Francisella tularensis subspecies tularensis, strain SCHU S4 in LPS biosynthesis, protein glycosylation, virulence and immunogenicity. One mutant, ∆wbtI, did not elaborate a long chain O-polysaccharide (OPS), was completely avirulent for mice, and failed to induce a protective immune response against challenge with wild type bacteria. Another mutant, ∆wbtC, produced a long chain OPS with altered chemical and electrophoretic characteristics. This mutant showed markedly reduced glycosylation of several known glycoproteins. Additionally this mutant was highly attenuated, and elicited a protective immune response against systemic, but not respiratory challenge with wild type SCHU S4. A third mutant, ∆kdtA, produced an unconjugated long chain OPS, lacking a detectable core structure, and which was not obviously expressed at the surface. It was avirulent and elicited partial protection against systemic challenge only.Keywords
This publication has 47 references indexed in Scilit:
- O-Linked Glycosylation of the PilA Pilin Protein of Francisella tularensis: Identification of the Endogenous Protein-Targeting Oligosaccharyltransferase and Characterization of the Native OligosaccharideJournal of Bacteriology, 2011
- Characterization of the O-antigen Polymerase (Wzy) of Francisella tularensis*Published by Elsevier BV ,2010
- A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuniProceedings of the National Academy of Sciences of the United States of America, 2010
- Multimethodological Approach to Identification of Glycoproteins from the Proteome of Francisella tularensis, an Intracellular MicroorganismJournal of Proteome Research, 2010
- Identification of an Essential Francisella tularensis subsp. tularensis Virulence FactorInfection and Immunity, 2009
- Cellular and humoral immunity are synergistic in protection against types A and B Francisella tularensisVaccine, 2008
- Identification ofFrancisella tularensis Himar1-Based Transposon Mutants Defective for Replication in MacrophagesInfection and Immunity, 2007
- A Defined O-Antigen Polysaccharide Mutant ofFrancisella tularensisLive Vaccine Strain Has Attenuated Virulence while Retaining Its Protective CapacityInfection and Immunity, 2007
- Role of the wbt Locus of Francisella tularensis in Lipopolysaccharide O-Antigen Biogenesis and PathogenicityInfection and Immunity, 2007
- The Immunologically Distinct O Antigens fromFrancisella tularensisSubspeciestularensisandFrancisella novicidaAre both Virulence Determinants and Protective AntigensInfection and Immunity, 2007