Designer functionalized self-assembling peptide nanofiber scaffolds for growth, migration, and tubulogenesis of human umbilical vein endothelial cells
- 10 September 2008
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Soft Matter
- Vol. 4 (12), 2388-2395
- https://doi.org/10.1039/b807155a
Abstract
We previously reported a class of designer self-assembling peptide nanofiber scaffolds as a unique biological material for diverse applications including 3-D tissue cell culture, slow drug release, regenerative medicine, and tissue engineering. One of these peptide scaffolds, RADA16-I has been used in bone, cartilage, and neural regeneration studies that have shown great promises. We here report the development of two new functionalized self-assembling peptide nanofiber scaffolds designed specifically for angiogenesis study through directly coupling pure RADA16-I with short biologically angiogenic motifs. Angiogenesis is very important in regenerative medicine. An adequate blood vessel supply to the newly formed tissue and within the transplanted scaffold is essential in determining the success of new tissue regeneration. In our study, two designer functionalized peptides, KLT, Ac–(RADA)4G4KLTWQELYQLKYKGI–CONH2 and PRG, Ac–(RADA)4GPRGDSGYRGDS–CONH2 significantly enhanced endothelial cell survival, proliferation, migration, and morphological tubulogenesis compared with unmodified RADA16-I scaffold. We also showed in our clear-boundary sandwich culture without adding extract soluble growth factors that cells migrated uni-directionally from RADA16-I toward the functionalized scaffolds but not the reverse. Our results suggest that the functionalized designer peptide scaffolds will not only have great promise for promoting endothelial cell growth, migration, and tubulogenesis, but also may have widely potential applications for diverse tissue engineering and tissues regeneration.Keywords
This publication has 41 references indexed in Scilit:
- Biological Designer Self-Assembling Peptide Nanofiber Scaffolds Significantly Enhance Osteoblast Proliferation, Differentiation and 3-D MigrationPLOS ONE, 2007
- Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional CulturesPLOS ONE, 2006
- Nano hemostat solution: immediate hemostasis at the nanoscaleNanomedicine: Nanotechnology, Biology and Medicine, 2006
- Slow release of molecules in self-assembling peptide nanofiber scaffoldJournal of Controlled Release, 2006
- Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of visionProceedings of the National Academy of Sciences of the United States of America, 2006
- Dynamic reassembly of peptide RADA16 nanofiber scaffoldProceedings of the National Academy of Sciences of the United States of America, 2005
- Fabrication of novel biomaterials through molecular self-assemblyNature Biotechnology, 2003
- Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repairProceedings of the National Academy of Sciences of the United States of America, 2002
- Self-complementary oligopeptide matrices support mammalian cell attachmentBiomaterials, 1995
- Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane.Proceedings of the National Academy of Sciences of the United States of America, 1993