Helicobacter pylori Adhesin Binding Fucosylated Histo-Blood Group Antigens Revealed by Retagging

Abstract

The bacterium Helicobacter pylori is the causative agent for peptic ulcer disease. Bacterial adherence to the human gastric epithelial lining is mediated by the fucosylated Lewis b (Le^b) histo-blood group antigen. The Le^b-binding adhesin, BabA, was purified by receptor activity–directed affinity tagging. The bacterial Le^b-binding phenotype was associated with the presence of the cag pathogenicity island among clinical isolates ofH. pylori. A vaccine strategy based on the BabA adhesin might serve as a means to target the virulent type I strains ofH. pylori.