Chondrocyte survival in cryopreserved osteochondral articular cartilage
- 1 May 1996
- journal article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 14 (3), 413-416
- https://doi.org/10.1002/jor.1100140311
Abstract
Fluorescent staining techniques were used in combination with confocal and conventional fluorescent microscopy to determine the location of viable chondrocytes in frozen and thawed osteochondral articular cartilage. The results showed that cell survival was confined to the superficial layer of the cartilage matrix. The addition of a cryopreservative agent (dimethyl sulfoxide) increased chondrocyte survival but only in the same layer. There was no evidence of cell survival in the middle or deep layers of the cartilage with or without the use of dimethyl sulfoxide. Under the conditions employed in this study, chondrocyte survival in a cryopreserved osteochondral allograft appears to be limited to the superficial layer of the articula cartilage.This publication has 7 references indexed in Scilit:
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