Development of a HPTLC method for in-process purity testing of pentoxifylline
- 1 April 2005
- journal article
- research article
- Published by Wiley in Journal of Separation Science
- Vol. 28 (6), 575-580
- https://doi.org/10.1002/jssc.200400006
Abstract
A HPTLC method for the separation and identification of pentoxifylline and related substances, impurities of reaction partners, and side reaction products has been developed using different mobile and stationary phases. For quantitative assay of possible by‐products as impurities, LiChrospher® RP‐18 F254s chromatoplates, acetone‐chloroform‐toluene‐dioxane (2:2:1:1 v/v) as a mobile phase, and detection at 275 nm were employed. Linearity (r ⪈ 0.997), recovery (86.5–115.5%), and determination limit (0.1–0.6%) were evaluated and found to be satisfactory. This method enables monitoring of the synthesis, as well as purity control of pentoxifylline‐containing raw materials and pharmaceuticals.Keywords
This publication has 11 references indexed in Scilit:
- Simple high-performance liquid chromatographic method for determination of pentoxifylline in human plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1998
- Determination of pentoxifylline in pharmaceutical formulations using iodine as oxidizing agentTalanta, 1998
- Separation and estimation of seven vasodilators using packed column supercritical fluid chromatographyJournal of Pharmaceutical and Biomedical Analysis, 1998
- Application of micellar electrokinetic chromatography to the quality control of pharmaceutical formulations: The analysis of xanthine derivativesElectrophoresis, 1994
- Determination of pentoxifylline and its metabolites in human plasma by high-performance liquid chromatography with solid-phase extractionJournal of Chromatography B: Biomedical Sciences and Applications, 1992
- Determination of pentoxifylline in serum by high-performance thin-layer chromatographyJournal of Pharmaceutical and Biomedical Analysis, 1991
- Study of the solid phase extraction of pentoxifylline and its major metabolite as a basis of their rapid low concentration gas chromatographic determination in serumBiomedical Chromatography, 1991
- Analysis of pentoxifylline in rabbit plasma using a Hisep™ high-performance liquid chromatography columnJournal of Chromatography B: Biomedical Sciences and Applications, 1990
- Determination of pentoxifylline and its 5-hydroxy metabolite in human plasma by solid-phase extraction and high-performance liquid chromatography with ultraviolet detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1989
- High-performance liquid chromatographic analysis of pentoxifylline and 1-(5'-hydroxyhexyl)-3,7-dimethylxanthine in whole bloodJournal of Chromatography B: Biomedical Sciences and Applications, 1987