Establishment and characterization of a primitive neuroectodermal tumor of bone continuous cell line (LAP‐35)

Abstract

A continuous tumor cell line (LAP‐35) was established from a primitive neuroectodermal tumor of bone from the right tibia of a 12‐year‐old female. The neural character of the cell line was documented by the spontaneous growth of neurites and by the presence of several neural markers, including neuron‐specific enolase (NSE), S‐100 protein, neurofilaments, chromogranin A, synaptophysin and positivity to monoclonal antibodies UJ127.11, UJ13A, UJ181.4. Cell‐sorter analysis showed a high expression of nerve growth factor receptor (NGFr) and major histocompatibility complex class I‐related molecules. A unique cytogenetic profile was observed, including a reciprocal chromosomal translocation (rct) 11:22 (q24;q12), typically associated with Ewing's sarcoma and neuroepithelioma, and deletion of the short arm of chromosome 1 (1p‐), otherwise a feature of neuroblastoma. N‐myc proto‐oncogene was neither amplified nor expressed, whereas the expression of c‐myc was documented by northern blot analysis. These features distinguish this new cell line from previously reported neuroectodermal cell lines, identifying LAP‐35 as a unique model of a group of neural bone tumors that share characteristics of neuroblastoma as well as neuroepithelioma.