Profiling and imaging proteins in the mouse epididymis by imaging mass spectrometry
- 27 October 2003
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 3 (11), 2221-2239
- https://doi.org/10.1002/pmic.200300474
Abstract
Different aspects of matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) have been used as discovery tools to obtain global and time-correlated information on the local proteomic composition of the sexually mature mouse epididymis from both qualitative and semiquantitative points of view. Tissue sections and laser captured microdissected cells and secretory products were analyzed by MALDI-MS and from the recovered protein profiles, over 400 different proteins were monitored. Over 50 of these, some of which have been identified, displayed regionalized behavior from caput to cauda within the epididymis. Combining the information obtained from high-resolution imaging mass spectrometry and laser captured microdissection experiments, numerous proteins were localized within the epididymis at the cellular level. Furthermore, from the signal intensities observed in the different protein profiles organized in space, semiquantitative information for each protein was obtained.Keywords
This publication has 46 references indexed in Scilit:
- GPX5 is present in the mouse caput and cauda epididymidis lumen at three different locationsMolecular Reproduction and Development, 2002
- Physiological functions and hormonal regulation of mouse vas deferens protein (AKR1B7) in steroidogenic tissuesChemico-Biological Interactions, 2001
- Probability-based protein identification by searching sequence databases using mass spectrometry dataElectrophoresis, 1999
- Molecular Cloning and Hormonal Regulation of a Murine Epididymal Retinoic Acid-Binding Protein Messenger Ribonucleic AcidEndocrinology, 1998
- Tissue and developmental distribution, dependence upon testicular factors and attachment to spermatozoa of GPX5, a murine epididymis-specific glutathione peroxidaseMolecular Reproduction and Development, 1997
- Mass spectrometry in protein studies from genome to functionCurrent Opinion in Biotechnology, 1997
- Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 1995
- Isolation, Immunolocalization, and Sperm-Association of Three Proteins of 18, 25, and 29 Kilodaltons Secreted by the Mouse Epididymis1Biology of Reproduction, 1992
- Specific Distribution of Messenger Ribonucleic Acids for 24-Kilodalton Proteins in the Mouse Epididymis as Revealed by in Situ Hybridization: Developmental Expression and Regulation in the Adult1Biology of Reproduction, 1991