A Novel Extraction Method Based on a Reversible Chemical Conversion for the LC/MS/MS Analysis of the Stable Organic Germanium Compound Ge-132
- 26 January 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 87 (4), 2042-2047
- https://doi.org/10.1021/ac504466u
Abstract
Poly trans-[(2-carboxyethyl)germasesquioxane] (IUPAC name) is the most common water-soluble organic germanium compound. This compound is known as bis(carboxyethyl)germaniumsesquioxide and it is commonly called Ge-132; it is hydrolyzed to 3-(trihydroxygermyl)propanoic acid (THGPA) in water. We have developed a method for the quantification of THGPA in rat plasma, using a novel extraction method based on a reversible chemical conversion. THGPA in plasma is converted to 3-(trichlorogermyl)propanoic acid (TCGPA) under acidic conditions using concentrated hydrochloride, which is followed by extraction with chloroform. TCGPA is then converted back to THGPA through hydrolysis. The extraction recovery of this method is approximately 100%. Moreover, we synthesized deuterated Ge-132, which was used as an internal standard in our experiments. This method covers a linearity range of 0.01–5 μg/mL for concentrations of THGPA in plasma. The intra-day and inter-day precisions of the analysis are about 4.1%, and the accuracy is within ±2.6% at THGPA concentrations of 0.025, 0.25, and 2.5 μg/mL. The total run time is 5 min. Our method was successfully applied to a pharmacokinetic investigation following oral administration of Ge-132.Keywords
This publication has 12 references indexed in Scilit:
- Analysis of Ge-132 and development of a simple oral anticancer formulationBioscience Horizons, 2011
- Induction of aminolevulinic acid synthase gene expression and enhancement of metabolite, protoporphyrin IX, excretion by organic germaniumEuropean Journal of Pharmacology, 2011
- Promotive Effects of the Dietary Organic Germanium Poly-trans-[(2-carboxyethyl) germasesquioxane] (Ge-132) on the Secretion and Antioxidative Activity of Bile in RodentsJournal of Health Science, 2010
- C-C Chemokine Receptor 2 Inhibitor Improves Diet-Induced Development of Insulin Resistance and Hepatic Steatosis in MiceJournal of Atherosclerosis and Thrombosis, 2010
- Inhibition of CCR2 Ameliorates Insulin Resistance and Hepatic Steatosis in db/db MiceArteriosclerosis, Thrombosis, and Vascular Biology, 2008
- Analytical product study of germanium-containing medicine by different ICP-MS applicationsJournal of Trace Elements in Medicine and Biology, 2004
- Propagermanium: a nonspecific immune modulator for chronic hepatitis BThe Esophagus, 2003
- Hazard Assessment of Germanium SupplementsRegulatory Toxicology and Pharmacology, 1997
- Chronic tubulointerstitial changes induced by germanium dioxide in comparison with carboxyethylgermanium sesquioxideKidney International, 1991
- Nephrotoxicity and neurotoxicity in humans from organogermanium compounds and germanium dioxideBiological Trace Element Research, 1991