Demonstration and partial characterization of the interferon‐gamma receptor on human B lymphocytes
- 1 August 1989
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 40 (4), 417-430
- https://doi.org/10.1002/jcb.240400403
Abstract
The expression of interferon-γ (IFN-γ) receptors on normal human B cells and four B cell lines was studied. Recombinant human IFN-γ was labeled with [γ- 32P]ATP using the catalytic subunit of a cAMP-dependent protein kinase. All four B cell lines, although differing in their responsiveness to IFN-γ, were found to express high-affinity receptors (1,000–11,000 receptors/cell). Normal unactivated B lymphocytes were also found to express constitutively high-affinity receptors, approximately 1,400 receptors per cell with an estimated affinity of 295 pM. Activation of the normal B cells in vitro with the polyclonal B cell activator, Staphylococcus aureus Cowan strain I (SAC), resulted in a slight decline in receptor number and a more pronounced fall in receptor density. One of the B cell lines and unactivated normal B cells were shown to internalize labeled IFN-γ rapidly. Chemical cross-linking of 32P-IFN-γ to the CB B cell line and to freshly isolated B lymphocytes revealed one major cross-linked receptor-ligand complex which had an estimated molecular weight of approximately 110 kilodaltons. This complex corresponded to a 93 kD receptor cross-linked to recombinant IFN-γ. Our data indicate that normal B lymphocytes constitutively express an approximately 93 kD IFN-γ receptor which is similar to the receptor present on Epstein-Barr virus-transformed B cell lines.Keywords
This publication has 40 references indexed in Scilit:
- Effect of tumor necrosis factor alpha on mitogen-activated human B cells.The Journal of Experimental Medicine, 1987
- Purification of human gamma interferon receptors by sequential affinity chromatography on immobilized monoclonal antireceptor antibodies and human gamma interferon.The Journal of Experimental Medicine, 1987
- Evidence for a gamma-interferon receptor that regulates macrophage tumoricidal activity.The Journal of Experimental Medicine, 1984
- γ-Interferon is one of several direct B cell-maturing lymphokinesNature, 1984
- Role of γ-interferon in antibody-producing responsesNature, 1984
- Regulation of B-Cell Growth and Differentiation by Soluble FactorsAnnual Review of Immunology, 1983
- Clonal differences in response to T cell replacing factor (TRF) for IgM secretion and TRF receptors in a human B lymphoblast cell lineEuropean Journal of Immunology, 1983
- T cell growth factor receptors. Quantitation, specificity, and biological relevanceThe Journal of Experimental Medicine, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949