High-fat feeding rapidly induces obesity and lipid derangements in C57BL/6N mice
Open Access
- 28 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Mammalian Genome
- Vol. 24 (5), 240-251
- https://doi.org/10.1007/s00335-013-9456-0
Abstract
C57BL/6N (B6N) is becoming the standard background for genetic manipulation of the mouse genome. The B6N, whose genome is very closely related to the reference C57BL/6J genome, is versatile in a wide range of phenotyping and experimental settings and large repositories of B6N ES cells have been developed. Here, we present a series of studies showing the baseline characteristics of B6N fed a high-fat diet (HFD) for up to 12 weeks. We show that HFD-fed B6N mice show increased weight gain, fat mass, and hypercholesterolemia compared to control diet-fed mice. In addition, HFD-fed B6N mice display a rapid onset of lipid accumulation in the liver with both macro- and microvacuolation, which became more severe with increasing duration of HFD. Our results suggest that the B6N mouse strain is a versatile background for studying diet-induced metabolic syndrome and may also represent a model for early nonalcoholic fatty liver disease.Keywords
This publication has 68 references indexed in Scilit:
- Hematological and acute-phase responses to diet-induced obesity in IL-6 KO miceCytokine, 2011
- Chronic subhepatotoxic exposure to arsenic enhances hepatic injury caused by high fat diet in miceToxicology and Applied Pharmacology, 2011
- Sequence-based characterization of structural variation in the mouse genomeNature, 2011
- Mouse genomic variation and its effect on phenotypes and gene regulationNature, 2011
- A conditional knockout resource for the genome-wide study of mouse gene functionNature, 2011
- Agouti C57BL/6N embryonic stem cells for mouse genetic resourcesNature Methods, 2009
- Cautionary insights on knockout mouse studies: The gene or not the gene?Brain, Behavior, and Immunity, 2009
- Fetal alcohol syndrome (FAS) in C57BL/6 mice detected through proteomics screening of the amniotic fluidBirth Defects Research Part A: Clinical and Molecular Teratology, 2008
- Characterization of the ethanol-deprivation effect in substrains of C57BL/6 miceAlcohol, 2006
- Initial sequencing and comparative analysis of the mouse genomeNature, 2002