A soluble form of CD83 is released from activated dendritic cells and B lymphocytes, and is detectable in normal human sera
Open Access
- 1 July 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 13 (7), 959-967
- https://doi.org/10.1093/intimm/13.7.959
Abstract
CD83 is an inducible glycoprotein expressed predominantly by dendritic cells (DC) and B lymphocytes. Expression of membrane CD83 (mCD83) is widely used as a marker of differentiated/activated DC but its function and ligand(s) are presently unknown. We report the existence of a soluble form of CD83 (sCD83). Using both a sCD83-specific ELISA and Western blotting, we could demonstrate the release of sCD83 by mCD83+ B cell and Hodgkin's disease-derived cell lines, but not mCD83– cells. Inhibition of de novo protein synthesis did not affect the release of sCD83 during short-term (2 h) culture of cell lines although mCD83 expression was significantly reduced, suggesting sCD83 is generated by the release of mCD83. Isolated tonsillar B lymphocytes and monocyte-derived DC, which are mCD83low, released only low levels of sCD83 during culture. However, the differentiation/activation of these populations both up-regulated mCD83 and increased sCD83 release significantly. Analysis of sera from normal donors demonstrated the presence of low levels (121 ± 3.6 pg/ml) of circulating sCD83. Further studies utilizing purified sCD83 and the analysis of sCD83 levels in disease may provide clues to the function and ligand(s) of CD83.Keywords
This publication has 31 references indexed in Scilit:
- Human acute myeloblastic leukemia cells differentiatein vitro into mature dendritic cells and induce the differentiation of cytotoxic T cells against autologous leukemiasEuropean Journal of Immunology, 1999
- Soluble and Membrane-bound Forms of Signaling Lymphocytic Activation Molecule (SLAM) Induce Proliferation and Ig Synthesis by Activated Human B LymphocytesThe Journal of Experimental Medicine, 1997
- B lymphocytes secrete antigen-presenting vesicles.The Journal of Experimental Medicine, 1996
- Regulation of ICAM‐3 (CD50) membrane expression on human neutrophils through a proteolytic shedding mechanismEuropean Journal of Immunology, 1994
- Protection from Fas-Mediated Apoptosis by a Soluble Form of the Fas MoleculeScience, 1994
- Circulating adhesion molecules in diseaseImmunology Today, 1993
- Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytesNature, 1992
- Membrane proteins with soluble counterparts: role of proteolysis in the release of transmembrane proteinsBiochemistry, 1991
- The Pi-linked receptor FcRIII is released on stimulation of neutrophilsNature, 1988
- Biotinylation: An alternative to radioiodination for the identification of cell surface antigens in immunoprecipitatesMolecular Immunology, 1987