High-definition spatial transcriptomics for in situ tissue profiling
Top Cited Papers
- 9 September 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 16 (10), 987-990
- https://doi.org/10.1038/s41592-019-0548-y
Abstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.Funding Information
- Knut och Alice Wallenbergs Stiftelse (2016.0454)
- Cancerfonden
- Stiftelsen för Strategisk Forskning
- Vetenskapsrådet
- European Molecular Biology Organization (ALTF 738-2017)
- Howard Hughes Medical Institute
- Klarman Family Foundation
- U.S. Department of Health & Human Services | National Institutes of Health (1OT2OD026673-1)
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (P2ZHP3_181475)
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