Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Top Cited Papers
- 1 October 2010
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 186 (2), 757-761
- https://doi.org/10.1534/genetics.110.120717
Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.Keywords
This publication has 20 references indexed in Scilit:
- High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleasesProceedings of the National Academy of Sciences of the United States of America, 2010
- Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzaeNew Phytologist, 2010
- Targeted genome editing in human cells with zinc finger nucleases constructed via modular assemblyGenome Research, 2009
- High-frequency modification of plant genes using engineered zinc-finger nucleasesNature, 2009
- Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)PLOS ONE, 2009
- Progress and prospects: Zinc-finger nucleases as gene therapy agentsGene Therapy, 2008
- Rapid “Open-Source” Engineering of Customized Zinc-Finger Nucleases for Highly Efficient Gene ModificationMolecular Cell, 2008
- Computer design of obligate heterodimer meganucleases allows efficient cutting of custom DNA sequencesNucleic Acids Research, 2008
- Os8N3 is a host disease-susceptibility gene for bacterial blight of riceProceedings of the National Academy of Sciences of the United States of America, 2006
- Chimeric Nucleases Stimulate Gene Targeting in Human CellsScience, 2003