Luteolin-rich artichoke extract protects low density lipoprotein from oxidationIn vitro
- 1 January 1998
- journal article
- research article
- Published by Informa UK Limited in Free Radical Research
- Vol. 29 (3), 247-255
- https://doi.org/10.1080/10715769800300281
Abstract
Flavonoids represent a diverse group of phytochemicals which possess the capacity to act as antioxidants in vitro. This study examined the free radical scavenging properties of a luteolin-rich artichoke extract and some of its pure flavonoid constituents by assessing their ability to prevent Cu2+-mediated LDL oxidation. Artichoke extract retarded LDL oxidation in a dose-dependent manner as measured by a prolongation of the lag phase to conjugated diene formation, a decrease in the rate of propagation and a sparing of endogenous LDL α-tocopherol during oxidation. The pure aglycone, luteolin (1 μM), demonstrated an efficacy similar to that of 20 μg/ml artichoke extract in inhibiting lipid peroxidation. Luteolin-7-O-glucoside, one of the glycosylated forms in the diet, also demonstrated a dose-dependent reduction of LDL oxidation that was less effective than that of luteolin. Studies of the copperchelating properties of luteolin-7-O-glucoside and luteolin suggest a potential role for chelation in the antioxidative effects of artichoke extract. Overall, the results demonstrate that the antioxidant activity of the artichoke extract relates in part to its constituent flavonoids which act as hydrogen donors and metal ion chelators, and the effectiveness is further influenced by their partitioning between aqueous and lipophilic phases.Keywords
This publication has 23 references indexed in Scilit:
- Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant propertiesBiochemical Journal, 1998
- Antioxidant flavonols and ischemic heart disease in a Welsh population of men: the Caerphilly StudyThe American Journal of Clinical Nutrition, 1997
- Human absorption and excretion of flavonoids after broccoli consumptionCancer Letters, 1997
- Structure-antioxidant activity relationships of flavonoids and phenolic acidsFree Radical Biology & Medicine, 1996
- Flavonoid intake and coronary mortality in Finland: a cohort studyBMJ, 1996
- Flavonoids as antioxidant agents: Importance of their interaction with biomembranesFree Radical Biology & Medicine, 1995
- Reduction of oxidative DNA-damage in humans by Brussels sproutsCarcinogenesis: Integrative Cancer Research, 1995
- Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries studyArchives of Internal Medicine, 1995
- Protective Effect of Epicatechin, Epicatechin Gallate, and Quercetin on Lipid Peroxidation in Phospholipid BilayersArchives of Biochemistry and Biophysics, 1994
- Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly StudyThe Lancet, 1993