Susceptibility of Mycobacterium tuberculosis Strains to Gatifloxacin and Moxifloxacin by Different Methods
- 1 June 2006
- journal article
- research article
- Published by S. Karger AG in Chemotherapy
- Vol. 52 (4), 190-195
- https://doi.org/10.1159/000093486
Abstract
Background: Considering the potentials of gatifloxacin and moxifloxacin in the treatment of tuberculosis, the present study was aimed to define resistance to both these drugs. Methods: Fifty Mycobacterium tuberculosis isolates, consisting of 30 ofloxacin-susceptible and 20 ofloxacin-resistant strains, were tested for their susceptibility to gatifloxacin and moxifloxacin using different susceptibility testing methods, namely the absolute concentration method on Lowenstein-Jensen medium (LJ), the proportion susceptibility testing method (PST) on LJ and 7H11 agar media, and the BACTEC radiometric method. Result: The minimal inhibitory concentration (MIC) of gatifloxacin and moxifloxacin was 1 µg/ml by the absolute concentration method on LJ. In the PST method on LJ and 7H11, using a criterion of ≧1% growth as resistant, there was 100% agreement with the absolute concentration method at a concentration of 0.5 µg/ml for gatifloxacin, and 96% agreement with the BACTEC method at a concentration of 0.25 µg/ml. For moxifloxacin, results by the PST method showed 96% agreement with the absolute concentration method on LJ at a concentration of 1 µg/ml and 92% agreement at a concentration of 0.5 µg/ml for both the absolute concentration method on 7H11 and the BACTEC method. Conclusions: The MICs of gatifloxacin and moxifloxacin were much lower than the MICs of other quinolones like ofloxacin and ciprofloxacin. Additionally, these two drugs have shown a low mean MIC and low concentration as a definition of resistance, which might help in treating the patients with low levels of quinolone resistance.Keywords
This publication has 16 references indexed in Scilit:
- Bactericidal Action of Gatifloxacin, Rifampin, and Isoniazid on Logarithmic- and Stationary-Phase Cultures of Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy, 2005
- Action of Fluoroquinolones and Linezolid on Logarithmic- and Stationary-Phase Culture of Mycobacterium tuberculosisChemotherapy, 2004
- Early Bactericidal Activity of Moxifloxacin in Treatment of Pulmonary Tuberculosis: a Prospective, Randomized StudyAntimicrobial Agents and Chemotherapy, 2004
- Moxifloxacin-containing Regimen Greatly Reduces Time to Culture Conversion in Murine TuberculosisAmerican Journal of Respiratory and Critical Care Medicine, 2004
- The Bactericidal Activity of Moxifloxacin in Patients with Pulmonary TuberculosisAmerican Journal of Respiratory and Critical Care Medicine, 2003
- Bactericidal Activity of Increasing Daily and Weekly Doses of Moxifloxacin in Murine TuberculosisAntimicrobial Agents and Chemotherapy, 2002
- In Vitro and In Vivo Activities of Gatifloxacin against Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy, 2002
- Pharmacokinetics of moxifloxacin, a novel 8‐methoxy‐quinolone, in patients with renal dysfunctionBritish Journal of Clinical Pharmacology, 2002
- Clinical Pharmacology of Gatifloxacin, a New FluoroquinoloneClinical Infectious Diseases, 2000
- TUBERCULOSIS AND HUMAN IMMUNODEFICIENCY VIRUS INFECTIONMedical Clinics of North America, 1997