Relative abundance, isolation and structure of phlebotomine microsatellites

Abstract

Popular classes of microsatellites are not always abundant in insects or easily isolated from them. Dot‐blot hybridizations demonstrated much variation in the relative abundance of four repeat classes in four phlebotomine sandfly species. Only AAT‐class repeats were specifically isolated from a phagemid library of Lutzomyia whitmani, even though other microsatellites had similar abundances. An enrichment step would have targeted classes but was omitted because relatively long flanking sequences were sought. All fourteen sandfly loci had a non‐coding structure, and a minority of dipteran AAT‐class repeats found in DNA databases and the literature were from exons. Therefore, this class should often provide neutral alleles for population studies. Perfect, not imperfect, AAT‐class repeats were polymorphic in wild L. whitmani.