Virus-Derived Gene Expression and RNA Interference Vector for Grapevine
Open Access
- 1 June 2012
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (11), 6002-6009
- https://doi.org/10.1128/jvi.00436-12
Abstract
The improvement of the agricultural and wine-making qualities of the grapevine ( Vitis vinifera ) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.Keywords
This publication has 58 references indexed in Scilit:
- Grapevine powdery mildew (Erysiphe necator): a fascinating system for the study of the biology, ecology and epidemiology of an obligate biotrophMolecular Plant Pathology, 2011
- Small RNA Duplexes Function as Mobile Silencing Signals Between Plant CellsScience, 2010
- Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis viniferaJournal of Virological Methods, 2009
- Development of a gene silencing DNA vector derived from a broad host range geminivirusPlant Methods, 2009
- Nidovirales: Evolving the largest RNA virus genomeVirus Research, 2006
- Diverse suppressors of RNA silencing enhance agroinfection by a viral repliconVirology, 2005
- Exploring plant genomes by RNA-induced gene silencingNature Reviews Genetics, 2003
- The present and future of the international wine industryNature, 2002
- Genetic Variation of Citrus Tristeza Virus Isolates from California and Spain: Evidence for Mixed Infections and RecombinationJournal of Virology, 2001
- Closterovirus Encoded HSP70 Homolog and p61 in Addition to Both Coat Proteins Function in Efficient Virion AssemblyVirology, 2000