Cooking Temperature Is a Key Determinant of in Vitro Meat Protein Digestion Rate: Investigation of Underlying Mechanisms
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- 28 February 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 60 (10), 2569-2576
- https://doi.org/10.1021/jf205280y
Abstract
The present study aimed to evaluate the digestion rate and nutritional quality of pig muscle proteins in relation to different meat processes (aging, mincing, and cooking). Under our experimental conditions, aging and mincing had little impact on protein digestion. Heat treatments had different temperature-dependent effects on the meat protein digestion rate and degradation potential. At 70 °C, the proteins underwent denaturation that enhanced the speed of pepsin digestion by increasing enzyme accessibility to protein cleavage sites. Above 100 °C, oxidation-related protein aggregation slowed pepsin digestion but improved meat protein overall digestibility. The digestion parameters defined here open new insights on the dynamics governing the in vitro digestion of meat protein. However, the effect of cooking temperature on protein digestion observed in vitro needs to be confirmed in vivo.Keywords
This publication has 38 references indexed in Scilit:
- Microstructural changes in m. rectus abdominis bovine muscle after heatingMeat Science, 2010
- Effect of cooking on protein oxidation in n-3 polyunsaturated fatty acids enriched beef. Implication on nutritional qualityMeat Science, 2010
- Changed dynamics in myofibrillar protein aggregation as a consequence of heating time and temperatureMeat Science, 2010
- Red meat consumption: An overview of the risks and benefitsMeat Science, 2010
- Use of meat fluorescence emission as a marker of oxidation promoted by cookingMeat Science, 2009
- Digestion study of proteins from cooked meat using an enzymatic microreactorMeat Science, 2009
- A Prospective Study of Red and Processed Meat Intake in Relation to Cancer RiskPLoS Medicine, 2007
- Use of a fluorescence front face technique for measurement of lipid oxidation during refrigerated storage of chicken meatMeat Science, 2007
- Pork of low technological quality with a normal rate of muscle pH fall in the immediate post-mortem period: The case of the Hampshire breedMeat Science, 1985