Biomimetic, Mild Chemical Synthesis of CdTe-GSH Quantum Dots with Improved Biocompatibility
Open Access
- 23 January 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (1), e30741
- https://doi.org/10.1371/journal.pone.0030741
Abstract
Multiple applications of nanotechnology, especially those involving highly fluorescent nanoparticles (NPs) or quantum dots (QDs) have stimulated the research to develop simple, rapid and environmentally friendly protocols for synthesizing NPs exhibiting novel properties and increased biocompatibility. In this study, a simple protocol for the chemical synthesis of glutathione (GSH)-capped CdTe QDs (CdTe-GSH) resembling conditions found in biological systems is described. Using only CdCl2, K2TeO3 and GSH, highly fluorescent QDs were obtained under pH, temperature, buffer and oxygen conditions that allow microorganisms growth. These CdTe-GSH NPs displayed similar size, chemical composition, absorbance and fluorescence spectra and quantum yields as QDs synthesized using more complicated and expensive methods. CdTe QDs were not freely incorporated into eukaryotic cells thus favoring their biocompatibility and potential applications in biomedicine. In addition, NPs entry was facilitated by lipofectamine, resulting in intracellular fluorescence and a slight increase in cell death by necrosis. Toxicity of the as prepared CdTe QDs was lower than that observed with QDs produced by other chemical methods, probably as consequence of decreased levels of Cd+2 and higher amounts of GSH. We present here the simplest, fast and economical method for CdTe QDs synthesis described to date. Also, this biomimetic protocol favors NPs biocompatibility and helps to establish the basis for the development of new, “greener” methods to synthesize cadmium-containing QDs.Keywords
This publication has 52 references indexed in Scilit:
- Design and Synthesis of Highly Luminescent Near-Infrared-Emitting Water-Soluble CdTe/CdSe/ZnS Core/Shell/Shell Quantum DotsInorganic Chemistry, 2009
- Simple and sensitive detection method for chromium(VI) in water using glutathione—capped CdTe quantum dots as fluorescent probesMicrochimica Acta, 2009
- Preparation of Highly Luminescent CdTe/CdS Core/Shell Quantum DotsChemphyschem, 2009
- Glutathione and Transition-Metal Homeostasis inEscherichia coliJournal of Bacteriology, 2008
- Synthesis and Bio-Imaging Application of Highly Luminescent Mercaptosuccinic Acid-Coated CdTe NanocrystalsPLOS ONE, 2008
- Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicityJournal of Artificial Organs, 2007
- Electrogenerated chemiluminescence from thiol-capped CdTe quantum dots and its sensing application in aqueous solutionAnalytica Chimica Acta, 2007
- Bacterial Toxicity of Potassium Tellurite: Unveiling an Ancient EnigmaPLOS ONE, 2007
- Catalases Are NAD(P)H-Dependent Tellurite ReductasesPLOS ONE, 2006
- A γ-irradiation reduction route to nanocrystalline CdE (E=Se, Te) at room temperatureMaterials Letters, 2003