Isolation and Characterization of Postnatal Stem Cells from Human Dental Tissues
Top Cited Papers
- 1 April 2007
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 13 (4), 767-773
- https://doi.org/10.1089/ten.2006.0192
Abstract
It was reported that postnatal stem cells are present in adult tissues such as bone marrow, liver, muscle, dental pulp, and periodontal ligament. We isolated postnatal stem cells from human dental tissues such as dental pulp (DPSC), periodontal ligament (PDLSC), periapical follicle (PAFSC), and the surrounding mandibular bone marrow (MBMSC) to ascertain their properties. Immunocytochemistry proved the existence of stem cells in these cell populations using STRO-1 as a stem cell marker. These cells also expressed the mesenchymal stem cell (MSC) markers CD29 and CD44. The isolated cells showed self-renewal capabilities and colony-forming efficiency. Almost all of the dental stem cells showed optimal growth when they were cultured in alpha modification of Eagle's medium (alpha-MEM) supplemented with 10% fetal calf serum (FCS) and 100 μM ascorbic acid. Only the PAFSC showed increased proliferation in 20% FCS and 50 μM ascorbic acid. All of the dental stem cells were capable of differentiating into adipocytes and mineral nodule forming cells. MBMSC, in particular, showed much better mineralization compared to the others. These results indicate that MSCs exist in various tissues of the teeth and can differentiate into osteoblasts, adipocytes, and other kinds of cells with varying efficiency.Keywords
This publication has 18 references indexed in Scilit:
- Engrafted Bone Marrow-Derived Flk-1+Mesenchymal Stem Cells Regenerate Skin TissueTissue Engineering, 2005
- Autogenous Injectable Bone for Regeneration with Mesenchymal Stem Cells and Platelet-Rich Plasma: Tissue-Engineered Bone RegenerationTissue Engineering, 2004
- The application of bone morphogenetic proteins to dental tissue engineeringNature Biotechnology, 2003
- Mechanisms of ectodermal organogenesisDevelopmental Biology, 2003
- The Evolving Concept of a Stem CellCell, 2001
- Bone Marrow Stromal Stem Cells: Nature, Biology, and Potential ApplicationsThe International Journal of Cell Cloning, 2001
- Reiterative signaling and patterning during mammalian tooth morphogenesisMechanisms of Development, 2000
- Stem CellsCell, 2000
- Localization of Putative Stem Cells in Dental Epithelium and Their Association with Notch and Fgf SignalingThe Journal of cell biology, 1999
- Signalling networks regulating dental developmentMechanisms of Development, 1997