Circulating Mononuclear Cells in the Obese Are in a Proinflammatory State
- 21 September 2004
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation
- Vol. 110 (12), 1564-1571
- https://doi.org/10.1161/01.cir.0000142055.53122.fa
Abstract
Background— In view of the increase in plasma concentrations of proinflammatory mediators tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP) in obesity, we investigated whether peripheral blood mononuclear cells (MNC) from obese subjects are in a proinflammatory state. Methods and Results— MNC were prepared from fasting blood samples of obese (n=16; body mass index [BMI]=37.7±5.0 kg/m 2 ) and normal-weight control (n=16; BMI=23.8±1.9 kg/m 2 ) subjects. Nuclear factor κB (NF-κB) binding to DNA in nuclear extracts was elevated ( P P P Conclusions— These data show (1) for the first time that MNC in obesity are in a proinflammatory state with an increase in intranuclear NF-κB binding, a decrease in IκB-β, and an increase in the transcription of proinflammatory genes regulated by NF-κB; (2) that plasma FFAs are a modulator of inflammation; and (3) that insulin resistance is a function of inflammatory mediators.This publication has 34 references indexed in Scilit:
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