Host Range of Small-Ruminant Lentivirus Cytopathic Variants Determined with a Selectable Caprine Arthritis- Encephalitis Virus Pseudotype System
- 15 August 2001
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (16), 7384-91
- https://doi.org/10.1128/jvi.75.16.7384-7391.2001
Abstract
The small-ruminant lentiviruses ovine maedi-visna virus (MVV) and caprine arthritis-encephalitis virus (CAEV) cause encephalitis, progressive pneumonia, arthritis, and mastitis in sheep and goats. Icelandic MVV strains, which are lytic in tissue culture, have a wide species distribution of functional receptors, which includes human cells. In contrast, functional receptors for the nonlytic CAEV CO are absent from human cells. To determine if the wide species distribution of functional receptors is a common property of MVV strains or related to cytopathic phenotype, we tested the infectivity of viruses pseudotyped with the envelope glycoproteins of MVV K1514, CAEV CO, and lytic and nonlytic North American MVV strains to cells of different species. Replication-defective CAEV proviral constructs lacking the env , tat , and vif genes and carrying the neomycin phosphotransferase gene in the vif - tat region were developed for the infectivity assays. Cotransfection of human 293T cells with these proviral constructs and plasmids expressing CAEV, MVV, or vesicular stomatitis virus envelope glycoproteins produced infectious pseudotyped virus which induced resistance of infected cells to G418. Using these pseudotypes, we confirmed the wide species distribution of Icelandic MVV receptors and the narrow host range of CAEV. However, functional receptors for the two North American MVV strains tested, unlike the Icelandic MVV and similar to CAEV, were limited to cells of ruminant species, regardless of cytopathic phenotype. The results indicate a differential receptor recognition by MVV strains which is unrelated to cytopathic phenotype.Keywords
This publication has 27 references indexed in Scilit:
- Properties of Wild-Type, C-Terminally Truncated, and Chimeric Maedi-Visna Virus Glycoprotein and Putative Pseudotyping of Retroviral Vector ParticlesJournal of Virology, 2001
- Envelope Glycoprotein Nucleotide Sequence and Genetic Characterization of North American Ovine LentivirusesVirology, 1997
- The CAEV tat Gene Trans-activates the Viral LTR and Is Necessary for Efficient Viral ReplicationVirology, 1993
- Radiation Hybrid Mapping: A Somatic Cell Genetic Method for Constructing High-Resolution Maps of Mammalian ChromosomesScience, 1990
- Pathogenesis of ovine lentivirus-induced arthritis: Phenotypic evaluation of T lymphocytes in synovial fluid, synovium, and peripheral circulationClinical Immunology and Immunopathology, 1989
- Nucleotide sequence of the visna lentivirus: relationship to the AIDS virusCell, 1985
- Characterization of the infection of caprine synovial membrane cells by the retrovirus caprine arthritis-encephalitis virusVirology, 1981
- Visna virus-induced fusion of continuous simian kidney cellsArchiv für die gesamte Virusforschung, 1974
- Plaque assay of visna virus using a secondary cellular overlay as an indicatorVirology, 1967
- GROWTH OF VISNA VIRUS IN PRIMARY TISSUE CULTURES FROM VARIOUS ANIMAL SPECIES1Acta Pathologica Microbiologica Scandinavica, 1962