MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a
Open Access
- 23 July 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 11 (1), 113-129
- https://doi.org/10.1007/s12575-009-9012-1
Abstract
MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based microRNA detection platform to identify genes that are induced by IFNα in hepatoma- or melanoma-derived human tumor cell lines. Despite the enormous differences in expression levels between these models, we were able to identify microRNAs that are upregulated by IFNα in both lines suggesting the possibility that interferon-regulated microRNAs are involved in the transcriptional repression of mRNA relevant to cytokine responses.Keywords
This publication has 20 references indexed in Scilit:
- Most mammalian mRNAs are conserved targets of microRNAsGenome Research, 2008
- The let-7 family of microRNAs2008
- MicroRNAs regulate critical genes associated with multiple myeloma pathogenesisProceedings of the National Academy of Sciences of the United States of America, 2008
- Highly sensitive and specific microRNA expression profiling using BeadArray technologyNucleic Acids Research, 2008
- lumi: a pipeline for processing Illumina microarrayBioinformatics, 2008
- Interferon modulation of cellular microRNAs as an antiviral mechanismNature, 2007
- SOCS proteins, cytokine signalling and immune regulationNature Reviews Immunology, 2007
- Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genesRNA, 2005
- MicroRNAs: Genomics, Biogenesis, Mechanism, and FunctionCell, 2004
- Expression modes of interferon-α inducible genes in sensitive and resistant human melanoma cells stimulated with regular and pegylated interferon-αGene, 2003