An improved colour reagent for the determination of blood glucose by the oxidase system

Abstract

A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase. The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.