Clean Substrates Prepared by Chemical Adsorption of Iodide Followed by Electrochemical Oxidation for Surface-Enhanced Raman Spectroscopic Study of Cell Membrane
- 20 May 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 80 (13), 5118-5125
- https://doi.org/10.1021/ac8003083
Abstract
Surface-enhanced Raman spectroscopy (SERS) has received renewed interest in recent years in fields such as trace analysis, biorelated diagnosis, and living cell study. However, the interference of impurities left on the surface from the preparation process of substrates or adsorbed from the ambient environment limits to some extent the application of SERS for analysis of trace or unknown samples. In the present paper, we propose a method to prepare clean SERS substrates by a combined method of chemical adsorption of iodide on the Au surface to remove the surface impurities and electrochemical oxidation of the adsorbed iodide to obtain a clean and impurity-free surface for SERS measurement. Time-dependent control experiment of untreated and treated substrate reveals that this method is very effective in obtaining substrates free of impurities. SERS mapping demonstrates the SERS activity of the substrate is homogeneous over the whole surface. The obtained clean substrate enables us to study the structures of the cell membrane with SERS and even to perform SERS mapping to visualize the distribution of amino acids over the membrane of a living cell.Keywords
This publication has 57 references indexed in Scilit:
- Designing, fabricating, and imaging Raman hot spotsProceedings of the National Academy of Sciences, 2006
- Surface-Enhanced Raman Scattering Studies of Human Transcriptional Coactivator p300The Journal of Physical Chemistry B, 2006
- Hot Spots in Silver Nanowire Bundles for Surface-Enhanced Raman SpectroscopyJournal of the American Chemical Society, 2006
- Part I: Surface-Enhanced Raman Spectroscopy Investigation of Amino Acids and Their Homodipeptides Adsorbed on Colloidal SilverApplied Spectroscopy, 2004
- Small Is Different: Shape-, Size-, and Composition-Dependent Properties of Some Colloidal Semiconductor NanocrystalsAccounts of Chemical Research, 2004
- Mass spectrometry-based proteomicsNature, 2003
- Iodine‐Induced Gold‐Nanoparticle Fusion/Fragmentation/Aggregation and Iodine‐Linked Nanostructured Assemblies on a Glass SubstrateAngewandte Chemie-International Edition, 2003
- Removal of Carbonaceous Contamination from SERS−Active Silver by Self-Assembly of DecanethiolAnalytical Chemistry, 1998
- Carbon Contamination at Silver Surfaces: Surface Preparation Procedures Evaluated by Raman Spectroscopy and X-ray Photoelectron SpectroscopyAnalytical Chemistry, 1996
- Probing redox-induced molecular transformations by atomic-resolution scanning tunneling microscopy: iodide adsorption and electrooxidation on gold(111) in aqueous solutionJournal of the American Chemical Society, 1992