Development of a 5′ Fluorogenic Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis
Open Access
- 1 February 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (2), 863-866
- https://doi.org/10.1128/jcm.41.2.863-866.2003
Abstract
A 5′ nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis . The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy.Keywords
This publication has 18 references indexed in Scilit:
- Identification of Actinobacillus actinomycetemcomitans Serotypes by Multiplex PCRJournal of Clinical Microbiology, 2001
- Formation of Methyl Mercaptan from l -Methionine by Porphyromonas gingivalisInfection and Immunity, 2000
- Prevalence of Specific Genotypes of Porphyromonas gingivalis fimA and Periodontal Health StatusJournal of Dental Research, 2000
- The role of Actinobacillus actinomycetemcomitans in the pathogenesis of periodontal diseaseTrends in Microbiology, 1997
- Real time quantitative PCR.Genome Research, 1996
- Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesionsOral Microbiology and Immunology, 1996
- Virulence factors ofActinobacillus actinomycetemcomitansrelevant to the pathogenesis of inflammatory periodontal diseasesFEMS Microbiology Reviews, 1995
- Identification of Actinobacillus actinomycetemcomitans: polymerase chain reaction amplification of IktA–specific sequencesOral Microbiology and Immunology, 1993
- Actinobacillus actinomycetemcomitans in human periodontal diseaseJournal of Clinical Periodontology, 1985
- Microbial Pathogenicity Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Virulence Factors in Colonization, Survival, and Tissue DestructionJournal of Dental Research, 1984