High-Throughput Sequencing of the Zebrafish Antibody Repertoire
- 8 May 2009
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 324 (5928), 807-810
- https://doi.org/10.1126/science.1170020
Abstract
Antibody Repertoire Revealed: Antibodies produced by B cells protect us against infection by a wide array of pathogens. Such wide-ranging responses are possible because the specific domain of the antibody that binds to the invader is highly variable owing to the somatic recombination of an inherited set of gene segments that encode the antibody molecule. Although antibody diversity within an individual organism is well-established, the specific antibody repertoire of an individual organism has not been characterized. Using high-throughput sequencing technology, Weinstein et al. (p. 807 ) characterized the diversity of the antigen-binding domain of the antibody heavy chain in zebrafish. The antibody repertoire of individual fish covered at least 50% of the possible gene combinations. Although the specific gene combinations differed between fish, a similar frequency distribution of the repertoire was observed. Unexpected examples of evolutionary conversion were also seen, with the same antibody observed in different animals.Keywords
This publication has 13 references indexed in Scilit:
- New perspectives for large-scale repertoire analysis of immune receptorsMolecular Immunology, 2008
- Reconstructing immune phylogeny: new perspectivesNature Reviews Immunology, 2005
- The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin ZNature Immunology, 2005
- Zebrafish as an immunological model systemMicrobes and Infection, 2002
- CDR3 length in antigen-specific immune receptors.The Journal of Experimental Medicine, 1994
- Estimating the Number of Species: A ReviewJournal of the American Statistical Association, 1993
- Somatic mutation: Diversity and regulation of the immune repertoireCell, 1987
- Isolation of a sulphate reductase-less mutant of the blue-green algaNostoc muscorumJournal of Basic Microbiology, 1978
- The Measurement of Species DiversityAnnual Review of Ecology and Systematics, 1974
- Immunological SpecificityScience, 1959