Retardation of C2C12 myoblast cell proliferation by exposure to low-temperature atmospheric plasma
- 18 July 2014
- journal article
- Published by Springer Science and Business Media LLC in The Journal of Physiological Sciences
- Vol. 64 (5), 365-375
- https://doi.org/10.1007/s12576-014-0328-5
Abstract
As the first step in evaluating the possibility of low-temperature atmospheric plasma for clinical applications in the treatment of rhabdomyosarcoma (RMS), we determined the effects of plasma exposure on C2C12 myoblasts. The low-temperature atmospheric plasma was generated through an electrical discharge in argon gas. One minute of plasma exposure every 24 h inhibited the cell proliferation, whereas myoblast differentiation was not affected. Plasma exposure increased the phosphorylation of ERK and JNK at 30 min after the exposure, but the phosphorylation of both was decreased to less than control levels at 1 and 4 h after the exposure. Plasma exposure increased the percentage of cells in the G2/M phase at 8 h after the exposure. In conclusion, plasma exposure retarded the proliferation of C2C12 myoblasts by G2/M arrest. Therefore, plasma exposure can be a possible treatment for the anti-proliferative effects of malignant tumors, such as RMS, without affecting differentiated skeletal muscle cells.Keywords
This publication has 37 references indexed in Scilit:
- Low‐temperature plasmas at atmospheric pressure: toward new pharmaceutical treatments in medicineFundamental & Clinical Pharmacology, 2013
- Biological Decontamination Using High and Reduced Pressure Nitrogen AfterglowsPlasma Processes and Polymers, 2011
- Effects of Non-Thermal Plasma on Mammalian CellsPLOS ONE, 2011
- RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen SpeciesAnnals of Biomedical Engineering, 2010
- Endothelial Cell Proliferation is Enhanced by Low Dose Non-Thermal Plasma Through Fibroblast Growth Factor-2 ReleaseAnnals of Biomedical Engineering, 2009
- Delayed Effects of Cold Atmospheric Plasma on Vascular CellsPlasma Processes and Polymers, 2008
- Platelet-Derived Growth Factor BB Induces Nuclear Export and Proteasomal Degradation of CREB via Phosphatidylinositol 3-Kinase/Akt Signaling in Pulmonary Artery Smooth Muscle CellsMolecular and Cellular Biology, 2006
- Activation of c‐Jun‐N‐terminal‐kinase is crucial for the induction of a cell cycle arrest in human colon carcinoma cells caused by flurbiprofen enantiomersThe FASEB Journal, 2003
- Cyclin-dependent kinase-1: linking apoptosis to cell cycle and mitotic catastropheCell Death & Differentiation, 2002
- WAF1, a potential mediator of p53 tumor suppressionCell, 1993