Effects of ethionine on the replicational fidelity in V79 Chinese hamster cells
- 1 July 1981
- journal article
- research article
- Published by Elsevier BV in Mutation Research
- Vol. 82 (2), 355-363
- https://doi.org/10.1016/0027-5107(81)90164-0
Abstract
The possibility that ethione, the ethyl analog of methionine and potent liver carcinogen, exerts its action by blocking the methylation of DNA and thereby rendering the post-replictive methylation instructed error-avoidance system inoperative was investigated. While the results are not directly supportive for the existence of such a repair system in V79 Chinese hamster cells, effects of ethionine were found. Following the exposure of ethionine-treated cells to EMS an increase in the cell killing and an decrease in mutation induction was observed. The base analog, 6-hydroxyaminopurine, was shown to be clearly mutagenic in the mammalian cells and in the presence of ethionine a drastic decrease in mutant frequency was observed. Ethionine itself did not appear mutagenic over the entire dose range tested (1–1000 μg/ml).This publication has 19 references indexed in Scilit:
- A new theory of carcinogenesisBritish Journal of Cancer, 1979
- Short-term tests for carcinogens and mutagensMutation Research/Reviews in Genetic Toxicology, 1979
- Effect of carcinogen ethionine on enzymatic methylation of DNA sequences with various degrees of repetitivenessEuropean Journal of Cancer (1965), 1979
- Spontaneous mutagenesis in Escherichia coli strains lacking 6-methyladenine residues in their DNA an altered mutational spectrum in dam− mutantsMutation Research, 1979
- Mutagenicity of thymidine to cultured Chinese hamster cellsNature, 1978
- Sequence specificity of the wild-type (dam+) and mutant (damh) forms of bacteriophage T2 DNA adenine methylaseJournal of Molecular Biology, 1978
- Use of restriction enzymes to study eukaryotic DNA methylationJournal of Molecular Biology, 1978
- Induced mutagenesis in dam − mutants of Escherichia coli: A role for 6-methyladenine residues in mutation avoidanceMolecular Genetics and Genomics, 1978
- Newly synthesised DNA is not methylatedBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- The occurrence of 6-methylaminopurine in deoxyribonucleic acidsBiochemical Journal, 1958