Comparison of 4GiardiaDiagnostic Tests in Diagnosis of Naturally Acquired Canine Chronic Subclinical Giardiasis
- 1 March 2010
- journal article
- research article
- Published by Wiley in Journal of Veterinary Internal Medicine
- Vol. 24 (2), 293-297
- https://doi.org/10.1111/j.1939-1676.2010.0475.x
Abstract
The performance of Giardia diagnostic tests that detect either cysts or fecal antigens has not been thoroughly examined. We examined the concordance and agreement among 4 Giardia diagnostic tests (2 cyst and 2 coproantigen detection methods) in a colony of dogs chronically and subclinically infected with Giardia. Twenty dogs with chronic, subclinical Giardia infection. Giardia diagnostic tests were performed repeatedly on each dog over 120 days. Fecal cyst detection methods (ZnSO4 flotation and fluorescent antibody [FAB] coproscopy) were performed 3 times per week. Coproantigen methods (Giardia SNAP test and quantitative ELISA) were performed weekly. Results were analyzed and compared among methods. When compared with FAB coproscopy, all of the in-house diagnostic tests had excellent positive predictive values (PPVs, 95-99%) at the study prevalence (89%). At lower prevalence rates, ZnSO4, SNAP, and ELISA tests all had good negative predictive values (NPVs), but poor PPVs. There was poor to good agreement among tests by kappa analysis. Our findings show that most commonly used in-house Giardia diagnostic tests have poor agreement with the gold standard method (FAB coproscopy). The in-house tests have good NPVs, but poor PPVs, at prevalence rates common in most clinical settings.Keywords
This publication has 14 references indexed in Scilit:
- A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogsVeterinary Parasitology, 2008
- Survey of giardiosis in household and shelter dogs from metropolitan areas of Curitiba, Paraná state, Southern BrazilVeterinary Parasitology, 2008
- Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of Cryptosporidium spp. and Giardia spp. in Naturally Exposed Cats in 4 Northern California Animal SheltersJournal of Veterinary Internal Medicine, 2007
- Genotyping of Giardia duodenalis from human and animal samples from Brazil using β-giardin gene: A phylogenetic analysisActa Tropica, 2007
- Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogsVeterinary Parasitology, 2007
- Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samplesClinical Microbiology & Infection, 2006
- Survey on giardiosis in shelter dog populationsVeterinary Parasitology, 2005
- Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardiaduodenalis and identification of potentially zoonotic subgenotypesInternational Journal for Parasitology, 2005
- Epidemiological and molecular evidence supports the zoonotic transmission ofGiardiaamong humans and dogs living in the same communityParasitology, 2004
- Evaluation of ColorPAC Giardia/Cryptosporidium Rapid Assay and ProSpecT Giardia/Cryptosporidium Microplate Assay for Detection of Giardia and Cryptosporidium in Fecal SpecimensJournal of Clinical Microbiology, 2001